Genetic Analysis of Digestive Physiology Using Fluorescent Phospholipid Reporters
- 18 May 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 292 (5520) , 1385-1388
- https://doi.org/10.1126/science.1060418
Abstract
Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.Keywords
This publication has 15 references indexed in Scilit:
- Biology, Pathology, and Interfacial Enzymology of Pancreatic Phospholipase A2Published by Springer Nature ,2001
- High Density Lipoprotein-mediated Cholesterol Uptake and Targeting to Lipid Droplets in Intact L-cell FibroblastsJournal of Biological Chemistry, 2000
- Secretory phospholipase Pla2g2a confers resistance to intestinal tumorigenesisNature Genetics, 1997
- The growing phospholipase A2 superfamily of signal transduction enzymesTrends in Biochemical Sciences, 1997
- The secretory phospholipase A2 gene is a candidate for the Mom1 locus, a major modifier of ApcMin-induced intestinal neoplasiaCell, 1995
- Chapter 6 Glycerophospholipid metabolismPublished by Elsevier ,1995
- Fluorescence-Based Assays of Lipases, Phospholipases, and Other Lipolytic EnzymesAnalytical Biochemistry, 1994
- Large-scale mutagenesis in the zebrafish: in search of genes controlling development in a vertebrateCurrent Biology, 1994
- Lipid dynamics in fish: aspects of absorption, transportation, deposition and mobilizationComparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1988
- Regional functional differentiation in the gut of the grasscarp, Ctenopharyngodon idella (Val.)Histochemistry and Cell Biology, 1979