High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry of phospholipids in Natronobacterium magadii
- 1 January 1999
- journal article
- Published by SAGE Publications in European Journal of Mass Spectrometry
- Vol. 5 (1) , 151
- https://doi.org/10.1255/ejms.262
Abstract
A novel method of coupled liquid chromatography/atmospheric pressure ionization mass spectrometry (LC/APCI-MS) has been used to analyze archaebacterium phospholipids in Natronobacterium magadii. Four major lipids, C20-C20 and C20-C25 PGP-Me and PG were found to exist in this organism. APCI mass spectra provided ample information for elucidation of the lipid structures. As an example, the positive-ion fragmentation of C20-C20 PGP-Me is discussed. In our study, LC/APCI-MS was shown to be an effective method in both the positive- and negative-ion modes for the direct mass spectrometric analysis of archaebacterium phospholipids.Keywords
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