Trachoma Viruses Isolated in United States. 1. Growth in Embryonated Eggs

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Abstract
From 6 active trachoma cases in the United States strains of“trachoma virus”have been isolated. Conjunctival scrapings suspended in broth-saline containing 1-10 μg/ml streptomycin were inoculated into yolk sac of embryonated eggs. From 1 to 6 blind passages were required for establishment of strains which subsequently reached titers of 106.3-107.4 egg LD50/ml. The essential nature of ill-defined egg“quality”for isolation of“trachoma viruses”is discussed. The isolated agents produced unequivocal eye infections in M. Cynomolgus and in a human volunteer.