Modulation of intercellular adherens-type junctions and tyrosine phosphorylation of their components in RSV-transformed cultured chick lens cells.
- 1 February 1991
- journal article
- Published by American Society for Cell Biology (ASCB) in Cell Regulation
- Vol. 2 (2) , 105-120
- https://doi.org/10.1091/mbc.2.2.105
Abstract
Transformation of cultured chick lens epithelial cells with a temperature-sensitive mutant of Rous sarcoma virus (tsRSV) leads to radical changes in cell shape and interactions. When cultured at the restrictive temperature (42 degrees C), the transformed cells largely retained epithelial morphology and intercellular adherens junctions (AJ), whereas on switch to the permissive temperature (37 degrees C) they rapidly became fibroblastoid, their AJ deteriorated, and cell adhesion molecules (A-CAM) (N-cadherin) largely disappeared from intercellular contact sites. The microfilament system that was primarily associated with these junctions was markedly rearranged on shift to 37 degrees C and remained associated mainly with cell-substrate focal contacts. These apparent changes in intercellular AJ were not accompanied by significant alterations in the cellular content of several junction-associated molecules, including A-CAM, vinculin, and talin. Immunolabeling with phosphotyrosine-specific antibodies indicated that both cell-substrate and intercellular AJ were the major cellular targets for the pp60v-src tyrosine-specific protein kinase. It was further shown that intercellular AJ components serve as substrates to tyrosine kinases also in nontransformed lens cells, because the addition of a combination of vanadate and H2O2--which are potent inhibitors of protein tyrosine phosphatases--leads to a remarkable accumulation of immunoreactive phosphotyrosine-containing proteins in these junctions. This finding suggests that intercellular junctions are major sites of action of protein tyrosine kinases and that protein tyrosine phosphatases play a major role in the regulation of phosphotyrosine levels in AJ of both normal and RSV-transformed cells.Keywords
This publication has 40 references indexed in Scilit:
- Cytoskeleton-associated cell contactsPublished by Elsevier ,2005
- Phosphotyrosyl protein phosphatasesBiochemical Journal, 1989
- Phosphotyrosine-modified proteins are concentrated at the membranes of epithelial and endothelial cells during tissue development in chick embryos.The Journal of cell biology, 1988
- Cell Transformation by the Viral src OncogeneAnnual Review of Cell Biology, 1987
- The Role of Changes in Cell Shape and Contacts in The Regulation of Cytoskeleton Expression During DifferentiationJournal of Cell Science, 1987
- Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous sarcoma virus.Proceedings of the National Academy of Sciences, 1986
- Phosphotyrosine-containing proteins are concentrated in focal adhesions and intercellular junctions in normal cells.Proceedings of the National Academy of Sciences, 1985
- Rous sarcoma virus-transformed fibroblasts adhere primarily at discrete protrusions of the ventral membrane called podosomesExperimental Cell Research, 1985
- Distribution of the cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture.The Journal of cell biology, 1985
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970