Lack of restriction at the blood-brain interface inLimulus despite atypical junctional arrangements

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Abstract
Summary Tracer and freeze-fracture techniques are used to evaluate the capacity of the central and peripheral nervous system of the horseshoe crab,Limulus polyphemus to admit or exclude molecular or ionic constituents of the blood intercellularly. Both the peripheral and central nervous systems are contained within blood sinuses so there is intimate contact between the haemolymph and the neural lamella. No discrete perineurium exists so any protection afforded to the nerve cells must be provided by the ensheathing glial cells and any junctions between them. Using ionic lanthanum as a pre-fixation incubation medium the system is seen to be completely ‘open’, with the tracer gaining access to all regions of the nervous tissue. Cellular associations in the peripheral nervous system, as revealed by thin-section and freeze-fracture, consist only of small scattered gap junctions between glial cells which afford no restriction to tracer entry. Gap junctions are again present between glial cells in the C.N.S. but here they are far more numerous, sometimes forming extensive sheets of almost continuous gap junctional plaques. Between certain glial cells there also exists a junctional system of linear PF ridges and complementary EF grooves; these may associate with or surround, often in discontinuous arrays, the gap junctional plaques. Given their characteristics and the freedom of tracer entry, they seem unlikely to represent either typical occluding tight junctions or septate junctions.