A COMPARATIVE COLOR TEST FOR COUMARIN AND MELILOTIC ACID IN MELILOTUS SPECIES

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Abstract
The coupling reaction between phenolic bodies and diazotized p-nitraniline in alkaline solution is shown to be of value in the detection and estimation of the coumarin and melilotic acid content of small quantities of sweet clover. The intensity of crimson color developed on treatment of pure coumarin solutions is shown to be proportional to the concentration of coumarin and to be adaptable to colorimetric measurements. For practical purposes it is shown that this reaction can be used for estimation of coumarin and melilotic acid together.It is possible to obtain the crimson color directly with plant extracts containing coumarin or melilotic acid, and a tentative method enabling comparisons to be made among small amounts of plant material is outlined. A comparison between results obtained by the colorimetric method outlined and by that of Obermayer shows that the latter method is open to several serious errors m the estimation of coumarin.

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