Nuclear division in isolated protoplasts from cells of higher plants grown in vitro
- 1 January 1969
- journal article
- Published by Springer Nature in Planta
- Vol. 89 (1) , 85-89
- https://doi.org/10.1007/bf00386499
Abstract
Protoplasts were isolated from cell suspensions of Haplopappus gracilis. The cell walls were degraded by the cellulase preparation “Onozuka P 1500” at a concentration of 5%. Sorbitol was found to work well as osmotic stabilizer in concentrations of 0.4–0.6 M. The protoplasts were cultured in growth medium after isolation; 3–5% went through nuclear division once and less than 1% also for a second time. No nuclear fusion was observed.Keywords
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