Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR)
- 23 January 2006
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 3 (2) , 91-93
- https://doi.org/10.1038/nmeth851
Abstract
We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces at atomic resolution.Keywords
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