Abstract
From tissue cultures (callus) of the soladulcidine strain of S. dulcamara L., the 4,4-dimethylsterols cycloartenol, cycloartanol, 24-dihydrolanosterol and 24-methylenecycloartanol and the sterols cholesterol, 24-methylenecholesterol, campesterol, stigmasterol, isofucosterol and sitosterol were identified by GC [gas chromatography] and GC/MS [mass spectroscopy]. In the callus, the sterols are present in the free form and as esters, glucosides and palmitic esters of the glucosides. The total contents and the contents of the combined forms were determined photometrically (total: 0.19% on a dry wt basis, 59.4% glucosides and acylated glucosides, 4.7% esters, 35.9% free). The main fatty acids of the petrolether soluble lipids of the callus (1.78%) are palmiltic-, linoleic- and linolenic acid.