BINDING OF CORTICOSTEROIDS BY PLASMA PROTEINS. VI. THE BINDING OF CORTISOL AND ALDOSTERONE BY CORTICOSTEROID-BINDING GLOBULIN AND BY THE ESTROGEN-INDUCED BINDING SYSTEM OF PLASMA*

Publisher Website
Google Scholar
Abstract
The binding of cortisol-4-C14 and aldosterone-H3 by serum from pregnant or estrogen-treated subjects was directly compared to the binding by serum from control subjects in a system of double equilibrium dialysis. Increased binding of cortisol-4-C14 could be demonstrated by "estrogen" plasma under conditions of minimal cortisol loading only at 37[degree]C. and not at 4[degree]C. This difference of binding behavior is evidence that the binding sites induced by estrogen are qualitatively different from those on normal corticosteroid-binding globulin. The total concentration of cortisol-binding protein induced by estrogens may be two or three times greater than that of corticosteroid-binding globulin. Under physiologic conditions of temperature, aldosterone-H3 is bound primarily by albumin, but at 4[degree] C. aldosterone-H3 is bound about equally by albumin and corticosteroid-binding globulin. At 4[degree] C. the estrogen-induced binding system does not have appreciable affinity for aldosterone-H .

This publication has 2 references indexed in Scilit: