Antibody to the inositol trisphosphate receptor blocks thimerosalenhanced Ca2+‐induced Ca2+ release and Ca2+ oscillations in hamster eggs
Open Access
- 7 September 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 309 (2) , 180-184
- https://doi.org/10.1016/0014-5793(92)81090-9
Abstract
The sulfhydryl reagent thimerosal enhanced the sensitivity of hamster eggs to injected inositol 1,4,5‐trisphosphate (InsP3) or Ca2+ to generate regenerative Ca2+ release from intracellular pools. A monoclonal antibody (mAb) to the InsP3 receptor blocked both the InsP3‐induced Ca2+ release (IICR) and Ca2+‐induced Ca2+ release (CICR). The mAb also blocked Ca2+ oscillations induced by thimerosal. The results indicate that thimerosal enhances IICR sensitized by cytosolic Ca2+, but not CICR from InsP3‐insensitive pools, and causes repetitive Ca2+ releases from InsP3‐sensitive pools.Keywords
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