Growth models of cultures with two liquid phases. VIII. Experimental observations on droplet size and interfacial area

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Abstract
Because of the importance of the drop she distribution and interfacial area of the dispersed liquid phase in hydrocarbon fermentations, experiments were carried out to determine the drop size distribution and the interfacial area during batch fermentations ofCandida lipolyticaon gas oil and onn‐hexadecane dissolved in dewaxed gas oil. The effects of cell concentration and dispersed phase volume fraction on size distribution and interfacial area were investigated. Measurements of interfacial tensions, densities, viscosities, and fatty acid concentrations were also made. The results show that the size distribution is skewed and that the Sauter mean diameter is in the range of 10 to 30 μ. Both the Sauter mean diameter and the interfacial area increased during the course of a batch fermentation; however, they decreased at the end of the fermentation. The interfacial area also increased with inoculum size.

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