Simplified generation of targeting constructs using ET recombination
- 1 September 1999
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 27 (17) , 16e-16
- https://doi.org/10.1093/nar/27.17.e16
Abstract
ET recombination is a way to engineer DNA in Escherichia coli using homologous recombination. Here we develop the potential of ET recombination in two ways relevant to complex engineering exercises such as building gene targeting constructs. First, a targeting construct was made in a single step. Second, ET recombination was used to place two unique restriction sites at precise positions in a large genomic clone. Subsequently a complex targeting construct was created by ligation with a multifunctional cassette.Keywords
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