Characterization of a new human lymphoma cell line (RC-K8) with t(11;14) chromosome abnormality

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Abstract
A human cell line (RC‐K8) that produces plasminogen activator was established from the peritoneal effusion of a patient with histiocytic lymphoma. The RC‐K8 cell line grew mainly in single cell suspension and consisted of primitive cells with pleomorphic morphology. RC‐K8 cells were positive for alpha‐naphthyl butyrate esterase, acid phosphatase and periodic acid‐Schiff stainings. Immunologic and molecular biological studies showed that RC‐K8 cells reacted with monoclonal antibodies to B cell antigens (B1 and Leul2) and la antigen (OKIa1) and possessed immunoglobulin gene rearrangement in the absence of surface and cytoplasmic immunoglobulin. Neither Epstein‐Barr virus nuclear antigen nor terminal de‐oxynucleotidyl transferees was detected in the cells. Chromosome analysis of RC‐K8 disclosed 46 XY with complex abnormalities including t(11;14)(q23;q32). Intraperitoneal inoculation of RC‐K8 cells to immunosuppressed newborn hamsters produced massive metastatic tumors in the lungs. The RC‐K8 cell line will be of considerable value for the study of lymphomagenesis associated with t(11;14) and pulmonary metastasis of lymphoma cells.