Research note: Detection of Salmonella in minced meat by the polymerase chain reaction method
Open Access
- 1 September 1995
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 21 (3) , 180-182
- https://doi.org/10.1111/j.1472-765x.1995.tb01036.x
Abstract
A specific PCR assay was used to detect Salmonella in enriched broths of 48 natural samples of minced pork and 48 natural samples of minced beef. By comparison with a routine culture method, the sensitivity of the PCR method was estimated to be 92% and the specificity of the PCR method was estimated to be 99%. The sensitivity of the culture method was estimated to be 50% in this study.Keywords
This publication has 12 references indexed in Scilit:
- Fluorescent detection-polymerase chain reaction (FD-PCR) assay on microwell plates as a screening test for salmonellas in foodsJournal of Applied Bacteriology, 1993
- Salmonella identification by the polymerase chain reactionMolecular and Cellular Probes, 1993
- Novobiocin, brilliant green, glycerol, lactose agar: a new medium for the isolation of Salmonella strainsResearch in Microbiology, 1992
- Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of SalmonellaMolecular and Cellular Probes, 1992
- Evaluation of the Magnetic Immuno PCR assay for rapid detection ofSalmonellaEuropean Journal of Clinical Microbiology & Infectious Diseases, 1991
- Immunomagnetic separation of Salmonella from foodsInternational Journal of Food Microbiology, 1991
- A Comparison of an Enzyme Immunoassay, DNA Hybridization, Antibody Immobilization, and Conventional Methods for Recovery of Naturally Occurring Salmonellae from Processed Broiler CarcassesJournal of Food Protection, 1991
- Non-radioactively labelled polynucleotide and oligonucleotide DNA probes, for selectively detecting Escherichia coli strains producing Vero cytotoxins VT1, VT2 and VT2 variantMolecular and Cellular Probes, 1991
- Isolation of a Salmonella‐specific DNA hybridization probeAPMIS, 1991
- The effect of incubation temperature and magnesium chloride concentration on growth of salmonella in home‐made and in commercially available dehydrated Rappaport‐Vassiliadis brothsJournal of Applied Bacteriology, 1989