Enzyme-Linked Immunosorbent Assay to Detect Potato Leafroll Virus in Potato Tubers and Viruliferous Aphids

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Abstract
Enzyme-linked immunosorbent assay (ELISA) with alkaline phosphatase successfully detected potato leafroll virus (PLRV) in naturally infected ''Russet Burbank'' potato tubers. Statistically significant differences in A405 [mean absorbance] values of ELISA reactions could be detected between known infected and healthy tuber samples. A multiple-eye sampling scheme was devised to detect PLRV in potato tubers by the ELIsA method. PLRV was also detected in viruliferous M. persicae by the ELISA method.