RAPID SCREENING FOR THE SPECIFIC DNA-SEQUENCE - ANALYSIS OF TRANSFORMING DNA SEGMENTS IN ADENOVIRUS-TRANSFORMED CELLS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 70  (2) , 239-243
Abstract
The viral DNA sequences in cells transformed by adenovirus type-12 (Ad12) DNA fragments were investigated by spot hybridization, a detection by autoradiography of nucleic acid hybrids formed between cell DNA spotted on a membrane filter and various nick-translated Ad12 DNA fragments. In CYl rat cells transformed by the EcoRI-C fragment (left hand 16%), all of the HindIII fragments included in the EcoRI-C fragment were present. In GYl rat cells transformed by the HindIII-G fragment (left hand 7%), the BpaI-H fragment and a part of the BpaI-J fragment, 2 components consisting of the HindIII-G fragment were found. A dominant presence of the Ad12 BpaI-H fragment (left hand 4.5%) of the Ad12 DNA molecule, approximately 60% of the Ad12 transforming DNA sequences (Ad12 HindIII-G), was also shown in GYl. Spot hybridization is useful for detecting viral nucleic acid sequences in cells and provides a simple and useful screening method for investigating the viral etiology of tumors and transformed cells.

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