Huntington's-chorea fibroblasts. Cellular protein glycosylation

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Abstract
Cell cultures (5) of Huntington''s chorea human skin fibroblasts exhibit greater than normal protein and lipid glycosylation when labeled with [U-14C]glucosamine. Oligosaccharide-polypeptide chains from all MW ranges are labeled differentially on sodium dodecyl sulfate/polyacrylamide-gel electrophoresis. This difference in protein glycosylation is not accompanied by any apparent difference in general cellular protein synthesis or by a differential rate of glucosamine uptake or decreased degradation of [14C]glycosylated macromolecules. Additionally [U-14C]glucosamine exclusively labels hexosamines and sialic acid of cellular macromolecules.