SAPONIFICATION AS A MEANS OF SELECTIVELY REVERSING THE METHYLATION BLOCKADE OF TISSUE BASOPHILIA
- 1 March 1959
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 7 (2) , 123-125
- https://doi.org/10.1177/7.2.123
Abstract
Saponification reverses the methylation blockade of cartilage and mast cell basophilia demonstrated by azure A above pH 3-4, but fails to do so at pH levels below this range. Saponification of oxidized hair cortex restores the methylation blocked basophilia demonstrable at pH 2 or higher. Mouse amyloid is characterized by a weak basophilia which is relatively resistant to methylation. The results are interpreted as indicating that methylation hydrolyzes the sulfate esters and esterifies other acid groupings in tissue sections.Keywords
This publication has 2 references indexed in Scilit:
- THE NILE BLUE REACTION OF PEPTIC GLAND ZYMOGEN GRANULES: THE EFFECT OF METHYLATION AND ALKALI DEMETHYLATIONJournal of Histochemistry & Cytochemistry, 1958
- CARTILAGE CHANGES IN PAPAIN-TREATED RABBITS1957