The Mutagenic Action of N-methyl-N#-nitro-N-nitrosoguanidine on Coprinus lagopus
- 1 January 1969
- journal article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 55 (1) , 121-125
- https://doi.org/10.1099/00221287-55-1-121
Abstract
SUMMARY: Optimal conditions, defined by the use of a known auxotrophic strain, are described for the mutagenesis of Coprinus lagopus oidia by N-methyl-N#-nitro-N-nitrosoguanidine (NTG), which is a very effective mutagen for bacteria and yeast. The oidia were best treated in phosphate buffer (pH 6.8) with a concentration of 15 μg. NTG/ml. for about 70 min. The yield of auxotrophs was considerably less than might be expected from a comparison of other systems in which NTG has been used as a mutagen.Keywords
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