An improved procedure for the isolation and purification of protoplasts from carrot suspension culture
- 1 January 1980
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 147 (4) , 283-286
- https://doi.org/10.1007/bf00379834
Abstract
A procedure is reported for the rapid and highly reproducible isolation of protoplasts from carrot suspension culture. The method utilizes Onozuka R 10 cellulase which has been purified by chromatography on Sephadex G75. Protoplast isolation, using this procedure, is quantitative and complete within 1 to 1.5 h. Intact protoplasts were separated from broken ones and other cellular debris by application of a polyethylene glycol/dextran two-phase system. The protoplasts isolated in this manner lack any detectable cell wall and are greater than 95% viable when assayed using fluorescein diacetate. It is concluded that such protoplasts are highly suitable for biochemical studies.This publication has 19 references indexed in Scilit:
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