Abstract
A method for the examination of hatchery fluff based on the determination of microbial counts was studied to determine the differences in counts calculated from 2 or more subsamples derived from a single specimen of fluff. From these data, 95% confidence limits were charted graphically. Also investigated was the effect on the counts of storage at room and refrigerator temperatures. Microbial counts derived from duplicate examinations were within confidence limits that can be satisfactorily related to a rating scheme devised by Wright. The specific microbial counts that designate ratings should be considered to be maximum figures. Storage of samples for periods up to 1 week did not result in appreciable deviations from the initial counts, and the differences could not be distinguished from normal variations observed within the confidence limits. It is concluded that this is a soundly based test that can be used with confidence for the study of hatchery sanitation in general and fumigation methods in particular. A summary of the results obtained in several laboratories revealed comparable percentages of satisfactory ratings in the various microbial categories.

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