Chlorantine Fast Green Bll as A Stain for Callose in Oak Phloem

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Abstract
Sections of oak bark were stained with chlorantine fast green BLL, rued u a 0.45% aqueous solution. All tissues were unstnined, except for local deposits of material associated with phloem cell walls, which stained deep green. This green-staining material also stained specifically with resorcinol blue and with the aniline blue fluorescence, technique, the usual histochemical tests for callose. The chlorantine fast green-staining material was removed from sections by treatment with a β-1,3-glucan hudrolase. It is concluded that chlorantine fast green BLL stains callose in plant sections and is a useful additional stain for the histochemical detection of this polymer.