Transxylosylation products of xylobiose by .BETA.-xylosidase-1 from Penicillium wortmanni IFO 7237.
- 1 January 1988
- journal article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 52 (5) , 1151-1158
- https://doi.org/10.1271/bbb1961.52.1151
Abstract
The transxylosylation reaction products of β-xylosidase-1, excreted by Penicillium wortmanni IFO 7237 using β-(1→4)-xylobiose as substrate, have been separated by chromatography on activated charcoal into four fractions, designated as P-1, P-2, P-3, and P-4, respectively. They were further purified by preparative paper chromatography. The characterization and structural analysis were done by measurement of the degree of polymerization (DP) and specific rotation followed by methylation analysis. Moreover, the enzymatic structural analysis of transxylosylation products, with high performance liquid chromatography (HPLC), allowed the confirmation of each structure. The first product, P-1, was β-(1→3)-xylobiose and the second, P-2, was β-(1→4)-xylotriose, but, P-3 was O-β-D-xylopyranosyl-(1→3)-O-β-D-xylopyranosyl-(1→4)-D-xylopyranose or isomeric xylotriose and P-4 was assumed to be O-β-D-xylopyranosyl-(1→4)-[O-β-D-xylopyranosyl-(1→3)]-O-β-D-xylopyranosyl-(1→4)-D-xylopyranose.Keywords
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