Identification of multiple ral gene products in human platelets that account for some but not all of the platelet Gn‐proteins
- 15 January 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 260 (1) , 48-52
- https://doi.org/10.1016/0014-5793(90)80063-o
Abstract
Polyclonal antibodies raised against specific recombinant low molecular mass GTP-binding proteins were tested for their ability to recognize partially purified human platelet membrane Gn-proteins (i.e. proteins that bind [α-32PlGTP on nitrocellulose blots of SDS/polyacrylamide gels). An antiserum against simian ralA protein recognized a 27 kDa human platelet protein with the same apparent molecular mass as the major platelet Gn-protein (Gn27). In further analysis by two-dimensional polyacrylamide gel electrophoresis, the isoelectric focusing step permitted resolution of 12 major Gn-protein forms, seven of 27 kDa (Gn27a-g), one of 26 kDa (Gn26) and four of 24 kDa (Gn24a-d). The ralA antibody reacted strongly with the five most basic Gn27 species (a-e), weakly with Gn26 and not at all with Gn27f, Gn27g or Gn24a-d. We conclude that ral gene products account for some but probably not for all of the platelet Gn-proteins.Keywords
This publication has 34 references indexed in Scilit:
- Multiple chromatographic forms of the formylpeptide chemoattractant receptor and their relationship to GTP-binding proteinsBiochemical and Biophysical Research Communications, 1989
- Characterization of G25K, a GTP-binding protein containing a novel putative nucleotide binding domainBiochemical and Biophysical Research Communications, 1989
- Purification of the 22 kDa protein substrate of botulinum ADP‐ribosyltransferase C3 from porcine brain cytosol and its characterization as a GTP‐binding protein highly homologous to the rho gene productFEBS Letters, 1989
- Identification of a platelet Mr 22,000 GTP-binding protein as the novel smg-21 gene product having the same putative effector domain as the ras gene productsBiochemical and Biophysical Research Communications, 1988
- GTPγS-stimulated hydrolysis of phosphatidyinositol-4,5-bisphosphate by soluble phospholipase C from human platelets requires soluble GTP-binding proteinBiochemical and Biophysical Research Communications, 1988
- Presence of guanine nucleotide-binding proteins in a plant hypocytyl microsomal fractionBiochemical and Biophysical Research Communications, 1988
- Identification of novel GTP‐binding proteins in the human neutrophilFEBS Letters, 1988
- A 29 kDa GTP-binding protein expressed in mouse brain, lung, kidney, spleen and transformed NIH3T3 cellsBiochemical and Biophysical Research Communications, 1987
- Iodogen‐catalyzed iodination of transferrinInternational Journal of Peptide and Protein Research, 1983
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976