CHARACTERIZATION OF HOST LYMPHOID CELLS IN ANTIBODY-FACILITATED BONE MARROW CHIMERAS
- 1 July 1985
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 40 (1) , 12-16
- https://doi.org/10.1097/00007890-198507000-00003
Abstract
We have produced stable murine antibody-facilitated (AF) chimeras by the simultaneous injection of Pi bone marrow cells and anti-P2 monoclonal antibody into normal (unirradiated) adult (P1XP2)F1 recipients. These AF chimeras are healthy, long-lived, and exhibit no overt signs of graft-versus-host disease. They are immunocompetent and tolerant of host, P2-encoded alloantigens. Donor cell engraftment and takeover, monitored by glucosephosphate isomerase isozyme patterns, is usually complete (≥95%) in the peripheral blood, bone marrow, and hemopoietic stem cell compartments of long-term (≥3 months posttransplantation) AF chimeras. We report here, however, that splenic, lymph node, and thymic leukocytes of AF chimeras represent donor/host chimeric populations. Spleen cell populations of AF chimeras exhibit substantial chimera-to-chimera variation in the preponderant residual host cell type(s) present. Our interpretations of the implications of these findings are discussed.This publication has 0 references indexed in Scilit: