Polypeptides of Infectious Bronchitis Virus. I. Polypeptides of the Virion

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Abstract
Infectious bronchitis virus (IBV) strain Beaudette grown in cultured [primary chicken embryo kidney] cells contained 5 structural proteins with apparent MW of 170,000 (p170), 94,000 (gp94), 50,000 (pp50) 30,000 (gp30) and 26,000 (p26). Both gp94 and gp30 are glycopeptides since they were labeled with[3H]glucosamine. The only phosphorylated polypeptide was pp50, and both it and gp94 were occasionally resolved into 2 bands. Two other polypeptides with MW of 28,000 (p28) and 14,000 (p14) were sometimes associated with the virus. In egg-grown virus 2 additional proteins were found with MW of 110,000 (p110) and 75,000 (gp75). The cell protein, actin, was also found in highly purified IBV virions. Different serotypes of either tissue culture-grown or egg-grown virus showed 1 of the 2 previously described distinct polypeptide patterns of IBV. Strain Beaudette gave a pattern characteristic of the M type; strain Connecticut gave a pattern characteristic of the C type. The polypeptides present in Connecticut virus were p170, gp98, pp50, gp28 and p26. The differences between the 2 patterns involve the mobility of the large (gp94/gp98) and small (gp30/gp28) glycopeptides.