The Estimation of Barbituric Acid Derivatives in Biological Material for Medico-Legal Purposes

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Abstract
Summary: A method is described for the estimation of barbiturates in biological material. The method avoids the extraction of substances giving undesirable blank values inherent in chloroform-sodium hydroxide extraction procedures. The method simplifies the spectrophotometric determination of those procedures using sodium hydroxide as the vehicle for the barbiturate in the spectrophotometer. The percentage recovery is about 90 per cent, and thus avoids, in forensic determinations, mathematical calculations for material which is not actually measured.

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