Confocal laser scanning fluorescence microscopy with a visible continuum source
- 1 January 2004
- journal article
- Published by Optica Publishing Group in Optics Express
- Vol. 12 (13) , 2844-2850
- https://doi.org/10.1364/opex.12.002844
Abstract
Confocal laser scanning fluorescence microscopy is demonstrated using a photonic crystal fiber-based excitation source. A 38 cm-long section of photonic crystal fiber is pumped with femtosecond pulses from a Ti:sapphire laser, and the resultant visible continuum is selectively filtered to provide the peak excitation wavelengths required for a range of fluorescently labeled biological tissue.Keywords
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