Abstract
Four species of fish from the genus Etheostoma, two species from the genus Percina, the yellow perch (Perca flavescens), and the walleye (Stizostedion v. vitreum), from the family Percidae, were examined karyotypically. All species had a diploid number of 48 chromosomes. Similarity in the karyotype of the yellow perch and walleye suggests a monophyletic origin for the subfamilies Percinae and Luciopercinae.Evolution in the family involved pericentric inversions and (or) translocations of chromosomes giving rise to metacentric and submetacentric chromosomes in the genera Percina and Etheostoma. Etheostoma exile, E. flabellare, and E. caeruleum all show a marked reduction in the size of the smallest pair of homologues (group C). The last two pairs of homologues in E. nigrum are of uniform size.

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