Detection and Quantification of Hepatitis C Virus (HCV) by MultiCode-RTx Real-Time PCR Targeting the HCV 3′ Untranslated Region
- 1 August 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (8) , 2635-2638
- https://doi.org/10.1128/jcm.02170-08
Abstract
A prototype, real-time reverse-transcription PCR assay, based on MultiCode-RTx technology, quantifying hepatitis C virus (HCV) RNA by targeting the HCV 3′ untranslated region demonstrated linearity over 7 logs, with a good correlation between the quantitative results of this assay and the results of two commercially available comparator assays for 466 clinical specimens comprising all six HCV genotypes.Keywords
This publication has 15 references indexed in Scilit:
- Improved COBAS TaqMan Hepatitis C Virus Test (Version 2.0) for Use with the High Pure System: Enhanced Genotype Inclusivity and Performance Characteristics in a Multisite StudyJournal of Clinical Microbiology, 2007
- Comparison of Performance Characteristics of Three Real-Time Reverse Transcription-PCR Test Systems for Detection and Quantification of Hepatitis C VirusJournal of Clinical Microbiology, 2007
- MultiCode-RTx Real-Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical SamplesJournal of Clinical Microbiology, 2006
- Multilaboratory Comparison of Hepatitis C Virus Viral Load AssaysJournal of Clinical Microbiology, 2006
- Multiplexed Detection of Anthrax-Related Toxin GenesThe Journal of Molecular Diagnostics, 2006
- Evaluation of the COBAS Hepatitis C Virus (HCV) TaqMan Analyte-Specific Reagent Assay and Comparison to the COBAS Amplicor HCV Monitor V2.0 and Versant HCV bDNA 3.0 AssaysJournal of Clinical Microbiology, 2005
- Performance Characteristics of a Quantitative TaqMan Hepatitis C Virus RNA Analyte-Specific ReagentJournal of Clinical Microbiology, 2004
- Verification of an Assay for Quantification of Hepatitis C Virus RNA by Use of an Analyte-Specific Reagent and Two Different Extraction MethodsJournal of Clinical Microbiology, 2004
- Nucleic Acid Analysis Using an Expanded Genetic Alphabet to Quench FluorescenceJournal of the American Chemical Society, 2004
- Assessing clinical utility of hepatitis c virus quantitative rt-PCR data: Implications for identification of responders among alpha-interferon-treated patientsMolecular Diagnosis, 1996