RECEPTOR BINDING PROPERTIES OF125I-hFSH PREPARED BY ENZYMATIC IODINATION
- 1 December 1974
- journal article
- other
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 39 (6) , 1159-1162
- https://doi.org/10.1210/jcem-39-6-1159
Abstract
Radioiodinated human follicle stimulating hormone (hFSH) has been prepared for receptor binding studies by lactoperoxidase catalysed iodination followed by purification on Sepharose-Concanavalin A. The 125I-hFSH tracer prepared by this method was relatively homogeneous on gel filtration and showed specific binding of 12% to excess of rat testis homogenate. Further purification of 125I-hFSH was performed by elution from particulate testis receptors at pH 5, with increase of specific binding to 28% during subsequent incubation with testis homogenate.Keywords
This publication has 0 references indexed in Scilit: