Blood glutathione peroxidase

Abstract
Manual and automated assays for the determination of glutathione peroxidase activity in bovine, sheep, pig, and human blood samples are described. The reactivation of the enzyme in blood samples after storage at −20°C was examined. Cyanide and Drabkin’s reagent, as used to prepare hemolysates in other assays, have been shown to inhibit enzyme activity, particularly in stored blood samples, and is omitted from this assay.