Subcellular Location of Progesterone in the Bovine Corpus Luteum: A Biochemical, Morphological and Cytochemical Investigation

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Abstract
The subcellular location of progesterone in the bovine corpus luteum has been investigated by biochemical, morphological and cytochemical techniques. Differential centrifugation of tissue homogenates showed that up to one-third of the total progesterone could be sedimented. Most of this particulate progesterone banded discretely at low density on sucrose gradients. Marker enzyme studies showed that it was not associated with either the microperoxisomes, lysosomes or mitochondria nor appreciably with the vesiculated subcellular components such as plasma membrane and microsomes.