Contribution of Nonautotrophic Carbon Dioxide Fixation to Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose

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Abstract
Bicarbonate-14C was provided to 5- and 11-day-old suspension cultures of Paul's Scarlet rose, and the incorporation of 14C into lipid, protein, amino acids, and organic acids was determined. The rate of bicarbonate uptake was approximately the same by 5- and 11-day-old cells, but the distribution of 14C among cell constituents was markedly different. In 5-day-old cells a larger proportion of the 14C entered protein, whereas in 11-day-old cells there was a greater tendency for 14C to accumulate in malate.