Silver-stained phenotyping of alpha 1-antitrypsin in dried blood and serum specimens.
Open Access
- 1 October 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 30 (10) , 1674-1676
- https://doi.org/10.1093/clinchem/30.10.1674
Abstract
In this technique for determining the electrophoretic phenotype of alpha 1-antitrypsin in dried blood or serum specimens, the adsorbed material is eluted with a concentrated solution of dithiothreitol, focused on polyacrylamide thin-layer gel, and made visible with silver stain. With this staining technique all normal and pathological alpha 1-antitrypsin phenotypes can be detected. The procedure is relatively simple, inexpensive, and suitable for use in large-scale screening for alpha 1-antitrypsin deficiency in selected populations.Keywords
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