BULK GROWTH PROCEDURES AND A BUTTON AGGLUTINATION-TEST FOR CAMPYLOBACTER

  • 1 July 1986
    • journal article
    • research article
    • Vol. 47  (7) , 1415-1418
Abstract
Bulk-cell yields were obtained from 4 Campylobacter spp incubated aerobically without the use of a special atmosphere. A button agglutination test was developed for quantitation of blood serum antibodies against C. fetus subsp venerealis, C. fetus subsp fetus, C. jejuni, and "C. hyointestinalis." The test was sensitive, and different individuals reading it usually attained the same titers. Cells of C. fetus subsp venerealis, C. fetus subsp fetus, and "C. hyointestinalis" grown aerobically in broth made satisfactory antigens for the button test, but some cell pools of C jejuni had a tendency to autoagglutinate. Cells of C. jejuni grown on blood agar had less tendency to autoagglutinate.

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