Proteasome function is required for activation of programmed cell death in heat shocked tobacco Bright‐Yellow 2 cells
- 6 February 2007
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (5) , 917-922
- https://doi.org/10.1016/j.febslet.2007.01.071
Abstract
To find out whether and how proteasome is involved in plant programmed cell death (PCD) we measured proteasome function in tobacco cells undergoing PCD as a result of heat shock (HS‐PCD). Reactive oxygen species (ROS) production, cytochrome c levels and caspase‐3‐like protease activation were also measured in the absence or presence of MG132, a proteasome inhibitor. We show that proteasome activation occurs in early phase of HS‐PCD upstream of the caspase‐like proteases activation; moreover inhibition of proteasome function by MG132 results in prevention of PCD perhaps due to the prevention of ROS production, cytochrome c release and caspase‐3‐like protease activation.Keywords
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