In Vivo Calcium Imaging of Circuit Activity in Cerebellar Cortex
Open Access
- 1 August 2005
- journal article
- research article
- Published by American Physiological Society in Journal of Neurophysiology
- Vol. 94 (2) , 1636-1644
- https://doi.org/10.1152/jn.01013.2004
Abstract
In vivo two-photon calcium imaging provides the opportunity to monitor activity in multiple components of neural circuitry at once. Here we report the use of bulk-loading of fluorescent calcium indicators to record from axons, dendrites, and neuronal cell bodies in cerebellar cortex in vivo. In cerebellar folium crus IIa of anesthetized rats, we imaged the labeled molecular layer and identified all major cellular structures: Purkinje cells, interneurons, parallel fibers, and Bergmann glia. Using extracellular stimuli we evoked calcium transients corresponding to parallel fiber beam activity. This beam activity triggered prolonged calcium transients in interneurons, consistent with in vitro evidence for synaptic activation of N-methyl-d-aspartate receptors via glutamate spillover. We also observed spontaneous calcium transients in Purkinje cell dendrites that were identified as climbing-fiber-evoked calcium spikes by their size, time course, and sensitivity to AMPA receptor antagonist. Two-photon calcium imaging of bulk-loaded cerebellar cortex is thus well suited to optically monitor synaptic processing in the intact cerebellum.Keywords
This publication has 39 references indexed in Scilit:
- Functional imaging with cellular resolution reveals precise micro-architecture in visual cortexNature, 2005
- Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivoNature Methods, 2004
- Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET ControlPLoS Biology, 2004
- A theory of cerebellar functionPublished by Elsevier ,2002
- Photon Upmanship: Why Multiphoton Imaging Is More than a GimmickNeuron, 1997
- Calcium transients in cerebellar granule cell presynaptic terminalsBiophysical Journal, 1995
- Subthreshold synaptic Ca2+ signalling in fine dendrites and spines of cerebellar Purkinje neuronsNature, 1995
- Morphology of parallel fibres in the cerebellar cortex of the rat: An experimental light and electron microscopic study with biocytinJournal of Comparative Neurology, 1994
- Principles of Organization of a Cerebro-Cerebellar CircuitBrain, Behavior and Evolution, 1981
- Fractured Somatotopy in Granule Cell Tactile Areas of Rat Cerebellar Hemispheres Revealed by Micromapping; pp. 94–105Brain, Behavior and Evolution, 1978