ISOZYMES OF ACID-PHOSPHATASE IN NORMAL AND CANCEROUS HUMAN PROSTATIC TISSUE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (11) , 4120-4124
Abstract
The supernatants of the homogenates from normal and cancerous human prostatic tissue run on polyacrylamide gel electrophoresis have 2 major electrophoretic bands when stained for prostatic acid phosphatase. The ratios of the electrophoretically distinguishable isoenzymes differ in normal and cancerous tissues. Similar distinctions between isoenzymes in normal and cancerous prostates are observed following column chromatographic separation or isoelectric focusing. The faster electrophoretic band can be separated by DEAE cellulose column chromatography or isoelectric focusing into at least 5 fractions with different electrophoretic mobilities. No differences were found in normal and cancerous tissues among these subfractions of the faster-moving electrophoretic band. Analysis by gel electrophoresis does not show association between these fractions after chromatographic or isoelectric separation of the prostatic acid phosphatase fractions. Quantitative, but not qualitative, differences in prostatic acid phosphatase isozymes occur in normal vs. cancerous prostates.

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