A Sensitive Method for Precise Measurement of Endogenous Angiotensins I, II&III in Human Plasma
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Clinical and Experimental Hypertension. Part A: Theory and Practice
- Vol. 9 (2-3) , 687-691
- https://doi.org/10.3109/10641968709164243
Abstract
We measured endogenous angiotensins (ANGs) I, II&III using a system of extraction by Sep-Pak column followed by high performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). An excellent separation of ANGs was obtained by HPLC. The recovery of ANGs I, II&III was 80–84%, when these authentic peptides were added to 6 ml of plasma. The coefficient of variation of the ANGs was 0.04–0.09 for intra-assay and 0.08–0.13 for inter-assay, thereby indicating a good reproducibility. Plasma ANGs I, II&III measured by this method in 5 normal volunteers were 51,4.5 and 1.2 pg/ml. In the presence of captopril, ANGs II&III decreased by 84% and 77%, respectively, while ANG I increased 5.1 times. This method is therefore useful to assess the precise levels of plasma ANGs.Keywords
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