The membrane-associated F0F1ATPase is essential for the viability ofStreptococcus pneumoniae
Open Access
- 1 June 2002
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 212 (1) , 133-138
- https://doi.org/10.1111/j.1574-6968.2002.tb11256.x
Abstract
Genetic studies aimed at eliminating expression of the atp operon (F0F1 H+-ATPase) of Streptococcus pneumoniae by genetic disruption of atpC, the first gene of the operon, with a chloramphenicol-resistance cassette were performed. Resistant transformants were obtained only when the recipient strain had a duplication of atpC, recombination occurring in such a way that transcription of the operon from its own promoter was allowed. These results imply that the atp operon is essential for the viability of the cells.Keywords
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