Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments
Open Access
- 1 July 1988
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (7) , 3301-3304
- https://doi.org/10.1128/jb.170.7.3301-3304.1988
Abstract
A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.Keywords
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