Metabolism of Separated Leaf Cells
- 1 July 1971
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 48 (1) , 14-18
- https://doi.org/10.1104/pp.48.1.14
Abstract
Enzymatically separated tobacco leaf cells took up amino acids, uracil, and uridine from the incubation medium and incorporated them into proteins and RNA, respectively, at a linear rate for approximately 30 hours. Both uptake and incorporation were light-dependent, although cells prepared from preilluminated plants or preillumination of cells allowed some uptake and incorporation to occur in the dark. The light was necessary to satisfy a photosynthetic requirement, but could be replaced in part by ATP in the medium.Keywords
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