The site of cleavage in infected cells and polypeptides of representative paramyxoviruses grown in cultured cells of the chorioallantoic membrane
- 1 March 1981
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 67 (1) , 19-30
- https://doi.org/10.1007/bf01314598
Abstract
Cultured cells of the chorioallantoic membrane (CAM) fulfilled the need of using the same cell system that was permissive for representative paramyxoviruses to carry out studies on the biosynthesis of their glycoproteins in infected cells. The polypeptide composition of the respective paramyxoviruses [Newcastle disease virus (NDV), paramyxovirus Yucapipa (PMY), and Sendai virus], grown in eggs and CAM-cells, was essentially identical. In egg-grown PMY a large glycoprotein (LGP) was present but only in some CAM-grown preparations of virus labeled with [3H]-glucosamine and rarely in [35S]-methionine or [3H]-amino acids (valine, leucine, and tyrosine) labeled viruses. The site of cleavage of precursor F0 to F1,2 was not the same. In contrast to the cleavage of Sendai virus glycoprotein, cleavage was intracellular in NDV and PMY infected cells. Homologous antisera against the glycoproteins failed to inhibit cleavage of HN0 or F0 in cells infected with the representative paramyxoviruses.This publication has 25 references indexed in Scilit:
- Electron microscope study of cultured cells of the chorioallantoic membrane infected with representative paramyxovirusesArchiv für die gesamte Virusforschung, 1980
- The Spread of a Pathogenic and an Apathogenic Strain of Newcastle Disease Virus in the Chick Embryo as Depending on the Protease Sensitivity of the Virus GlycoproteinsJournal of General Virology, 1979
- Glycoproteins of representative paramyxoviruses: Isolation and antigenic analysis using a zwitterionic surfactantJournal of Medical Virology, 1978
- Two disulfide-linked polypeptide chains constitute the active F protein of paramyxovirusesVirology, 1977
- Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virusVirology, 1976
- Glycoproteins of Sendai Virus: Purification and Antigenic AnalysisIntervirology, 1975
- Effect of specific antibodies on biological functions of the envelope components of Newcastle disease virusVirology, 1974
- Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virusVirology, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Functional significance of sialidase during influenza virus multiplicationVirology, 1966