The regulation of the proton conductance of brown fat mitochondria
- 19 September 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 161 (2) , 284-288
- https://doi.org/10.1016/0014-5793(83)81026-6
Abstract
The binding of purine nucleotides to intact brown fat mitochondria is re-examined. In addition to the previously reported high affinity binding site, a low-affinity site is found, which requires several minutes to saturate. Only the high affinity site is functional in regulating the proton and halide permeabilities of the mitochondria. The low affinity site can introduce errors in the use of nucleotide binding to quantitate the M r 32 000 uncoupling protein unique to these mitochondria.Keywords
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