Isolation and characterization of somatostatin from pigeon pancreas.

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Abstract
Most of the somatostatin-like activity from pigeon pancreas corresponded to a small species with an apparent MW of 1500-2500. This species was isolated under conditions minimizing intermolecular interactions and protease activities. The isolated product was characterized by 2 somatostatin radioimmunoassays, a bioassay, endgroup determination and amino acid analysis. The structure of the isolated compound was determined to be H-Ala-Gly-cyclo-(Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys)-OH. Additionally, small amounts of des-Ala1-somatostatin, a possible degradation product of pancreatic somatostatin, and a large somatostatin-like species with an apparent MW of 11,000-12,500 were detected. The main somatostatin-like polypeptide isolated from pigeon pancreas is identical to the mammalian hypothalamic tetradecapeptide somatostatin.