Isolation of Leptospira grippotyphosa from a Cow Following an Abortion

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Abstract
Summary L. grippotyphosa was isolated from the urine of a cow 7 days after abortion. The isolant grew poorly in Stuart's liquid medium and Fletcher's semisolid medium. Experimental semisolid and liquid media containing bovine albumin fraction V and Tween-80 proved valuable as isolation and growth media, Gerbils and hamsters were more susceptible than guinea pigs and white mice to the newly isolated organism. Serologic evidence indicates L. grippotyphosa is widely distributed in Illinois cattle and swine. A special acknowledgement is due to Dr. A. D. Alexander and Mr. L. B. Evans, Walter Reed Army Inst. of Research, Washington, D. C., for confirmation of the identity of the isolated organism. The authors also wish to acknowledge the assistance provided by Dr. W. Lovett, Geneva, Ill.