Characterization of the RNA Mycovirus Infecting Allomyces arbuscula

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Abstract
The mycovirus infecting A. arbuscula was fractionated into 2 particle classes designated A and B. Particles A have a buoyant density of 1.385 g/cm3, in CsCl, a sedimentation coefficient of 75S and contain 1 major and 2 minor polypeptides with MW of 34,000, 31,000 and 28,000, respectively. Particles B band at 1.335 g/cm3, sediment with a value of 67S and, in addition to the 3 polypeptides as in particles A, they contain 7 extra polypeptides, 6 of which are of lower molecular weights. Particles A and B contain double-stranded RNA which was resolved by electrophoresis on polyacrylamide gels into 3 components with MW of 1.4 (major), 2.5 and 1.1 .times. 106 (minor). Both particles also contain single-stranded RNA. Particles A contain 27% RNA whereas particles B only contain 14%. Particles A and B apparently belong to the same type of virus and represent 2 different replicative stages.

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