Estimation of Ionic Concentrations and Intracellular pH in Slices from Different Areas of Rat Brain

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Abstract
Tissue spaces occupied by14C‐sucrose and3H‐inulin were measured at different incubation time intervals (15–120 min) in rat brain slices prepared from cortex, cerebellum, caudate, diencephalon and midbrain. Total water content, total Na+and K contents and14C‐DMO (5,5‐dimethyl‐2,4‐oxaolidinedione) spaces were obtained from the same tissues. At all time intervals,14C‐sucrose spaces were larger than corresponding3H‐inulin spaces. After 60 min incubation, the mean values for14C‐sucrose spaces in preparations from different brain areas ranged from 31.5 to 38.1 m1/100 g, whereas the3H‐inulin space mean values were 19.3–26.1 m1/100 g. Average intracellular pH was calculated from14C‐DMO spares in the presence and absence of 3.67 mM procaine. The pH1values obtained using inulin‐derived extracellular spares were 6.91–6.99, and those calculated from sucrose spaces were 6.77–6.89. The contents of K+and Na+showed no distinct regional differences, but a potassium uptake with time could only be observed in preparations from cerebral and cerebellar cortex.